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Thermophilic fungi are the chief components of the microflora that develops in heaped masses of plant materials, piles of agricultural and forestry products and other accumulation of organic matter. In this investigation, survey was conducted and collected different compost samples from eight localities of Osmanabad district. Among these localities, Naldurg and Dhoki sites showed highest number of organic substrates, while least in Itkal. Physicochemial properties i.e. nitrogen, phosphorus, potassium, total organic matter, total carbon and moisture were analysed from available composts and among them nitrogen was highest in molasses. Isolation of fungi was made from substrates and among five substrates, vermicompost and Farm Yard Manure (FYM) were recorded for highest species richness and % incidence while less in molasses. Among isolated thermophilic fungi, Aspergillus niger, A. flavus, Rhizoctonia solani, A. fumigatus and Rhizopus sp. found abundant in all the substrates and temperature ranges. Fungal species A. niger, Mucor mucedo, A. flavus and Pythium sp. Highest growth on all three media viz. Potato dextrose agar (PDA), Czapek’s Dox Agar (CZA) and Martins Rose Bengal (MBR) were recorded. A. niger and Rhizopus sp. were found dominant in tested media and temperature. Generally, the fungi show very little growth at 65 °C. Every temperature gradient showed the growth incidence in all substrates but room temperature (RT) and 35 °C showed richness of fungal incidence. Even some species showed the growth on some substrates at higher temperate (65 °C) to some extent.

Clitoria ternatea L. is an excellent herbal medicinal plant. Arbusular Mycorrhizal fungal (AMF) colonization and biomass of three different tested conditions of Clitoria ternatea plant was investigated. Inoculums of indigenous AMF and Trichoderma harzianum was tested greenhouse experiment and compared with natural condition after 60th days of treatment. Percentage of Arbuscular mycorrhizal (AM) infection, number of resting spores and AM fungi species varies in different land. Among three different conditions, natural conditions showed maximum root colonization (75.89%) than treated one but minimum spore density (358.8/100gsoil). Highest spore density (481.6/100g soil) was found in T. harzianum treated condition followed by indigenous AMF treatment Acaulospora Glomus and Sclerocystis these three genera were found frequently. AMF inoculums and T.harzianum treatments conditions were observed promising biomass data of 60th days after treatment (DAT).When AMF are more colonized to plants then enhanced the biomass productivity.

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Present investigation describes that the study site comes under Aurangabad Division Maharashtra and it falls in Deccan Plateau Zone of India. It was collected different types of organic substrates viz. vermiompost, poultary manure, baggase, farm yard manure (FYM), soil, Ash etc. Isolated thermophilic predominant fungi thermophilic fungi viz.Aspergillus niger, Mucor mucedo,Humicola  insolens,Trichoderma harzianum,T. viride,Penicillium duponti,Fusarium oxysporun and Chaetomium thermophilum were carried out for the production of enzymes. Isolated predominant thermophilic fungi were evaluated on different types of enzymes. Among tested thermophilic fungi, the highest ativity was observed in C. thermophilium (20mm)  followed by T. harzianum (19.50mm) In lipase, M. mucedo  (15.40mm) was found maximum followed by F. oxysporun. Cellulase activity was found highest in A. nige (25mm) followed by others. In case of xylanase, catalase, peroxidase  and esterase activities were found maximum, minimum  and medium even negative in some fungi. Maximum pectinase activity was detected from H. insolens (52.26 @ 0 min) and (74.25 @ 10 min) and in case of M. mucedo, F. oxysporun and C. thermophilium was found most extreme while least in A. niger (30.12) and P. duponti (33.47) @ 0 minute.   Key words: Organic Substrates, Thermophilic Fungi, Enzymes