Context:Probiotics are living microorganisms, principally bacteria, that are safe for human consumption and, when ingested in sufficient quantities, have beneficial effects on human health, beyond basic nutrition. However, in India people are not aware of their benefits and availability.Aims:To find status of probiotics in India and to assess the awareness regarding probiotics among chemists in New Delhi, India.Settings and Design:A cross-sectional survey was conducted among 300 chemist shops in different areas of New Delhi, India.Materials and Methods:Data were collected through a structured questionnaire, which was responded by the chemist in the course of a telephonic interview. The questionnaire addressed on the availability of probiotic products at their chemist shop.Statistical Analysis Used:The data so gathered were subjected for descriptive analysis using t test.Results:As per availability of probiotic products, total of 25 probiotic products were available at different chemist shops. None of the chemist shop had all the 25 products available. 10% shops had 75% products available and there were 5% such shops which didn’t have any of the products available.Conclusion:Efforts should be made to increase the awareness among chemists about the probiotic products.