Molecular biology techniques (dot hybridization and polymerase amplification of DNA) are used to diagnose cytomegalovirus infection in pregnant women and newborns. It is concluded from the results that dot hybridization is a convenient tool in screening studies and that polymerase DNA amplification followed by verification of the reaction product using restriction enzymes and blot hybridization makes the diagnosis of this disease more reliable and may fred wide application in practical laboratories.
The data of populational and hospital cancer registers, cytological and immunological laboratories of Saint-Petersburg are examined. More than 350 new invasive cervical cancer cases are registered annually. Since 1970 up to 1991 oncological morbidity decreased from 14,7 to 8,1 per 100000 women, but then we witness steady rise of standardized indeces up to 9,9 in 1999. Increasingrate of so called neglectedforms of cervical cancer (III-IV stages), especially amongyoung women, is a disturbing fact. The issues of prophylaxis and early diagnostics of cervical cancer are discussed in the article. The results of cytological and virological examination of women of different contingents are drawn.
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