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PurposeThe purpose of this paper is to study bio‐safe technology – impact of rhizospheric bacteria Azotobacter and Bacillus within the composition of environmentally clean complex microbiological biofertilizer “BioWais” prepared on the basis of nitrogen‐, phospho‐ and silicon‐bacterin on physiological parameters of plants: growth, productivity, the role of rhizobacteria in plants resistance to biotic and abiotic factors.Design/methodology/approachThe following contemporary methods of investigation of physiological parameters of biological objects: immunoenzyme analysis of phytohormones, method of combinatory light dissemination, determination of amino acid composition of grain proteins by ion‐exchange chromatography, spectrophotometric methods were used.FindingsThe positive impact of biofertilizer “BioWais” on plant growth and development, enhancement of resistance of bacterized plants to hypothermia and pathogenic infection, increase of carotid content were demonstrated.Research limitations/implicationsThe bacteria under study biopreparation were shown to act as plant growth‐promoting rhizobacteria foster increase of plant anti‐oxidant protection, adaptation of seedlings to hypothermia, synthesize cytokinins and indolyl acetic acid.Practical implicationsBacterial preparation increases quality and amount of agricultures yield, it may be recommended to treat seeds prior to planting with the view to stimulate germination, to enhance plants resistance to hypothermia and fungus‐induced diseases, for improvement of plant growth and development.Originality/valueNew, innovative, environmentally pure bacterial preparation BioWais showed significant efficiency for a number of agrocultures and may be used for acquisition of safe plant products of high quality (with high content of protein, essential amino acids and carotin, potential source of vitamin A, low content of nitrates).