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By coupling an immobilized enzyme system with an electrochemical sensor, the reagent requirement for this glucose method is eliminated. Miniaturization and a further simplification of the instrumentation for the continuous analysis of glucose is achieved.

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LITERATURE CITED(1) Am. Soc. Testing Materials, Philadelphia, Pa.j "Methods for Emission Spectrochemical Analysis," Designation E-115-59T, p. 1, and E-116-59T, p. 12 (1960).

The method is based on the coupled enzyme reaction, in which oxidation of glucose i s catalyzed specifically, with the subsequent formation of a colored reaction product. A few seconds after the. start the rate of formation of the colored reaction product i s proportional to glucose concentration, and this rate can b e measured accurately and automatically within about 1 minute after the reaction begins. An instrument was assembled to measure precisely the extremely small change of absorbm c e that occurs during the first part of the glucose reaction. A time ratio is obtained which is directly proporiional to concentration. A few commercially available basic units and an easily constructed control system and enzyme injector are put together so that the glucose measurement is rapid and simple. Automatic results for the determination of glucose in standards and blood serum show Good proportionality and a co2fficient of variation of about 2%.