An overview of state of art in computerized object recognition techniques regarding digital images is revised. Advantages of shape based techniques are discussed. Importance of -Fourier Descriptor‖ (FD) for the shape based object representation is described. A survey for the available shape signature assignment methods with Fourier descriptors is presented. Details for the design of shape signature containing the crucial information of corners of the object are depicted. A novel shape signature is designed basing on the Farthest Point Angle (FPA) which corresponds to the contour point. FPA signature considers the computation of the angle between the line drawn from each contour point and the line drawn from the farthest corner point. Histogram for each 15o angle conceiving the information of the object is constructed. FPA signature is evaluated for three standard databases; viz., two in Kimia {K-99, K-216} and one in MPEG CE-1 Set B. The performance of the present FPA method estimated through recognition rate, time and degree of matching and is found to be higher.

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Plants have an amazing ability to cope with wide variety of stresses by regulating the expression of genes and thus by altering the physiological status. In the past few years, canonical microRNA variants (isomiRs) have been shown to play pivotal roles by acting as regulators of the transcriptional machinery. In the present research, we present Diff isomiRs, a web-based exploratory repository of differential isomiRs across 16 sequenced plant species representing a total of 433 datasets across 21 different stresses and 158 experimental states. Diff isomiRs provides the high-throughput detection of differential isomiRs using mapping-based and model-based differential analysis revealing a total of 16,157 and 2,028 differential isomiRs, respectively. Easy-to-use and web-based exploration of differential isomiRs provides several features such as browsing of the differential isomiRs according to stress or species, as well as association of the differential isomiRs to targets and plant endogenous target mimics (PeTMs). Diff isomiRs also provides the relationship between the canonical miRNAs, isomiRs and the miRNA-target interactions. This is the first web-based large-scale repository for browsing differential isomiRs and will facilitate better understanding of the regulatory role of the isomiRs with respect to the canonical microRNAs. Diff isomiRs can be accessed at: www.mcr.org.in/diffisomirs.

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