The present study focused on the isolation and identification of fungal endophytes from Talinum fruticosum. Endophytic fungi live inside the higher plants apparently without causing harm. Talinum fruticosum has medicinal value and is a good source of vitamins A and C, an excellent source of calcium and phosphorus. Leaves and stems of Talinum fruticosum were evaluated to isolate endophytic fungi from Talinum fruticosum collected from different regions of Mysuru, Karnataka, India. All isolates of fungal species were identified based on morphological characteristics, mycelium, asexual spores, and sexual spores observe under the compound microscope. 20 species were isolated from leaves, and 5 fungal species were isolated and identify fungal species from leaves are Rhizopus sp, Aspergillus sp, Penicillium sp, Rhizopus stolonifera, Aspergillus sp, Fusarium sp, and sterile hyphae. 3 fungal species were unidentified. And 20 species were isolated from the stems and 6 fungal species were isolated and identify the fungal species from stems were Fusarium sp, Curvalaria sp, Aspergillus sp, Rhizopus stolonifera, Colletotrichum sp, Trichoderma sp, Sterile hyphae. 5 fungal species were unidentified.