Background: Cervical cancer is one of the most common cancers among women worldwide and its rates are higher in low and middle income countries. India is one not exemplified from the developing countries. Aims: The main aim of the present work is a preliminary prospective screening study to observe the incidence of human papilloma virus (HPV) virus in the specimens of cervical biopsy taken from the patients of gynecology department of Visakha Steel General Hospital, RINL, Visakhapatnam district. Screening procedures like Pap smear, Colposcopy Immunohistochemistry (IHC) were done to identify the prevalence of HPV. It is an effort to assess and also improving the quality of cervical cancer prevention and treatment services in Visakhapatnam district of North coastal Andhra Pradesh. Settings and design: This prospective study was conducted at the department of obstetrics and gynaecology, Visakha Steel General Hospital, RINL, Visakhapatnam district from January 2010 to December 2010. Materials and methods: The Pap test and colposcopic examination were conducted on 161 patients and IHC was done on 24 cervical biopsy patients. Results: The 87.58% of women was observed with abnormal Pap smear among that the severe dysplasia is around 10%. The abnormal cervix was seen by colposcopy is 89.44%. The 24 squamous metaplasia cases were subjected to IHC and 8 cases were found to be positive. Conclusion: Pap smear, colposcopy along with cervical biopsy for HPV can provide enough information for an accurate diagnosis of cervical cancer and also from the future course of action for the treatment of the patients.