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Photoreactive derivatives of E. coli tRNAPhe bearing arylazido groups on guanine residues (azido-tRNA) were used for affinity labelling of E. coli ribosomes in the region of the P-site when the A-site was either free or occupied by aminoacyl-or peptidyl-tRNA.Corresponding complexes of azido-tRNA with ribosomes and poly(U) were obtained both nonenzymatically and with the use of elongation factors. UVirradiation of the complexes resulted in labelling of ribosomal proteins (preferentially of 30 S subunit). Proteins S9 and S21 were labelled only when the A-site was free; S14 -only when it was occupied; Sl 1,

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Eukaryotic initiation factor eIF-2 is an oligorneric protein consisting of three different subunits. During initiation of protein synthesis eIF-2 interacts with GTP, Met-tRNAland 40 S ribosomal subunit. By afftnity labeling with a photo-reactive GTP analogue it was shown that in the binary complex [eIF-2. GTP] GTP is in contact with the y-subunit of eIF-2.