The research aimed to illustrate enzyme level such as liver and immune enzymes to detect toxic effect of chemical agriculture pesticides as biological indictors to chemical pesticides contamination. 102 laboratory mice were used as a model 20-25gm weight. 30 mice were used to determine LD50 which was 30 mg/mouse. Mice were divided in to three groups according to LD50. After exposed to three different concentration by three ways (Interaperitoneal,Oraly,and Spray). Results showed significant increased in enzymes levels of (ALP), (GOT), (GPT), and (ADA) after 7 days of the three different exposure, while after 14 days there was decreased in enzymes levels of (ADA), (GOT), and(ALP) with increases in (GPT) enzyme: according to use "interaperitoneal injection and spray method'', that companied by the appearance of gross pathological lesions in mice organs represented by hypertrophy of liver and there is many cysts filled with pus which related with increased in pesticide concentration and duration of exposure in addition to bile duct obstruction to some exposed group. The results show the possibility of the adoption of variation in the level of enzymes to give an idea of the type and the impact of the pesticide, which was subjected to the animals.