The review presents data on chemical-analytical methods of PCDD, PCDF determination and biotesting methods used for toxicological assessment of pollutants. Distribution of PCDDs, PCDFs on the territory of Moscow is analyzed. Analysis of publications of Russian and foreign authors showed that the currently existing methods of biotesting are in fact untested for dioxins. In order to quickly establish the toxicological effect of persistent organic pollutants in soil, it is suggested to use biotesting methods, with inclusion of test organisms representing the main trophic levels of ecosystems: producers, consumers, and decomposers.
The paper considers the influence of polychlorinated dibenzo-p-dioxins, dibenzofurans, biphenyls and benz[a] pyrene on the germination of barley seeds, as well as the influence of pollutants on seed storage. As a result of the experiments, the negative effect of polychlorinated biphenyls on seed germination at a concentration of 100 μg/ kg has been found, which can be explained by the high accumulation of low-chlorinated pollutants by seeds. The negative effect of benz[a]pyrene on seed germination at concentrations of 20 and 100 μg/kg has been registered.
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