Aim: The present study was undertaken to ascertain the prevalence and drug sensitivity pattern of causative agents of mastitis in and around Namakkal, Tamilnadu, India. Materials and Methods:A total of 74 milk samples were collected and bacterial isolates from 72 samples were obtained and subjected to in-vitro chemotherapeutic sensitivity test.Results: Out of 72 samples, 32 (44.44%) were found positive for Staphylococci species, 4 (5.5%) for Streptococci species, 30 (41.66%) for E.coli and 6 (8.33%) for Klebsiella species. The drug sensitivity test performed irrespective of isolates, revealed sensitivity of organisms to various antibiotics and none of the isolates were found to be sensitive to penicillin G. Conclusion:Enrofloxacin was found to be more effective antibiotic among all the tested antibiotics.

The present study was undertaken to determine the prevalence of leptospires in field rats in and around laboratory animal facilities in Bangalore. 34 rats were trapped alive in and around the laboratory animal facilities in Bangalore. Urine and serum samples from theses field rats were collected. Serum samples were tested for anti-leptospiral antibodies by microscopic agglutination test, while urine samples were subjected for dark field microscopy and polymerase chain reaction to detect the presence of leptospiral antigens. Serology revealed the presence of antileptospiral antibodies in 19 (61.29 percent) field rats and dark field microscopy revealed the presence of leptospiral antigens in 3 (8.82 percent) and 6 (17.65 percent) of urine samples of these field rats. Among the serovars, Icterohaemorrhagiae was predominant followed by Autumnalis and Pyrogens. Serology dark field microscopy and polymerase chain reaction reveals that field rats are major natural carriers and shedders of leptospires.