The article is devoted to the study of the relationship between emotional intelligence, empathy and tolerance to uncertainty and their role in the formation of the managerial potential of managers in agricultural enterprises. The high level of uncertainty and risks that accompany the management process in agro-industrial enterprises places increased demands on the level of managerial competencies development in their managers. The development level of highly professional managers is one of the important factors for agribusiness, because the quality of management depends on the company’s effectiveness. The managerial potential of the manager, along with the personnel policy and management system of the organization, plays a leading role in the formation of the agro-industrial enterprise managerial potential. Managers with high emotional intelligence and tolerance to uncertainty are much easier to survive crises, which contributes to the development of the right management decisions and, as a result, the economic success of the enterprise. There were significant differences in the scales of expression control, tolerance to uncertainty, empathic fantasy, empathic distress, and empathic care for female and male managers.