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The objective of this study was the development of a method for the quantification of free fatty acids (FFA) using less aggressive reactants against the handler and the environment than those used in the classic method of Lowry and Tinsley. The modified procedure is a variation of the Lowry and Tinsley method employing cyclohexane in place of benzene. The use of benzene is prohibited in certain work processes and laboratories, and the competent authority in each country is actively promoting research into harmless or less harmful products that could replace benzene. A comparison with the traditional AOCS titration method for oil analysis was performed. FFA content in mackerel frozen at -10 degrees C was measured according to the three methods over a 12 month period. The results showed similar values, and good correlations were obtained.

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Salmon slices (Salmo salar) were packed under CO2 and stored in a chill room at 2±1°C for 3 weeks. A study was carried out on the effect of CO2 atmosphere on bacterial growth as well as on chemical deterioration (pH, total volatile bases, (TVB), trimethylamine (TMA), lipid oxidation) and sensory features (exudation, raw fish odour, cooked fish flavour). Results were compared with a control stored in air. Salmon slices stored under CO2 had a shelf‐life of nearly twice as long as those in air, having lower bacterial counts, pH, TMA and TVB, as well as improved sensory features after 18 days at 2±1°C. Exudation was not significantly different between control and CO2‐stored samples after 10 days ice storage. Exudate values extended from 1 to 2% for CO2‐stored samples after 10 and 20 days storage, respectively. Salmon slices can be stored under CO2 at 2±1°C for about 18 days, with no substantial loss of quality. Shelf‐life can vary depending upon the state of fish, handling practices, initial bacterial load and storage temperature.