Paraguay is integrated into the world mainly through its agricultural activity. The population’s perception of genetically engineered crops is relevant to design communication strategies that convey the advantages and limitations of the various technologies used in the country. We aimed to know the perception of the population of four Departments of the country where such crops are grown through a survey, which revealed a low level of knowledge about genetically engineered crops in general, and specifically about the effects of genetically engineered crops on production, nutrition, and the environment. Respondents expressed a willingness to receive information on genetically engineered crops, in particular from the National Government and the Health Sector .