Helicobacter pylori is the major causative agent of Gastric carcinoma. Significance of the urease accessory interaction proteins are emphasized in colonization of human gastric mucosa and efficient infection of H. pylori. Here an attempt is made to explore the structure and properties of urease accessory interaction proteins from Helicobacter pylori J 99. The proteins chosen for the study are ureH, ureI, nikR, groL and flgS based on the interaction map available from STRING database. The above mentioned proteins do not have a comprehensive three dimensional structure. Hence the models were generated using PSI-BLAST (Position Specific Iterative-Blast) and MODELLER 9V8. Physicochemical characterization encompasses pI, EC, AI, II and GRAVY. Secondary structure was predicted using PSI-PRED. Functional characterization was done by SOSUI and DISULFIND Servers and refinement of structure was done using Ramachandran plot analysis. RMS-Z values were calculated using Q-MEAN Server and CHIMERA was used for molecular simulation studies. Plant defensins from Vigna radiata are successfully docked to the modeled structures and thus interaction could be possibly prevented. These results will pave way for further selective inhibition of H. pylori colonization and in vivo survival by employing plant defensins from Vigna radiata (VrD1 & VrD2). The work will prove that plant defensins provides anticancer relief too.

The physicochemical characterization of seven marine sediment samples was investigated and collected from different zones along with Thondi coastal sediments along the southeast coast of India. The study found that variation in physical parameters and mud contents were due to differences in sediment source. Results of Station 3 revealed the calculated average values as follows: Bulk density (BD) (1.3 g/cm 3 ), particle size distribution of -sand (50.5%), silt (31.5%), and clay (18%). Further, the water holding capacity (WHC) was observed to be 65.3%. These studies moderately describe the WHC of space or pores of the soil particles. The fine, well sored sediments contain BD, particle size distribution, and water holdinsg capacity. According to the chemical characterization, sediments were categorized into organic carbon (OC) (1.2%) and organic matter (OM) (2.0%) the area for land derived materials from surrounding rocks. Moderate significant linear correlations were observed between OC and TN, indicating the occurrence of these components in a common phase organic matter. Even though, in the correlation of the total phosphate in organic matter, these two elements was negatively correlated, indicating anthropogenic regions of phosphate exportation. The study found three well structured sediments of the bacterial community association and primary productivity.