The comparative phytochemical analysis of leaves, stem and fruit powder of Tribulus terrestris was carried out by using preliminary phytochemical screening. To identify the phytochemical constituents, present in water and ethanol extracts of Tribulus terrestris leaves, stem and fruit, a preliminary analysis was done by using different testing methods of Frothing test, Mayer’s test, Hager’s test, Foam formation test, Lead acetate test, Molisch’s test and Ferric Chloride test. The phytochemical analysis study showed the presence of 3.903%, 1.725% and 2.259% terpenoids & phenolic in leaves, stem and fruit parts of Tribulus terrestris respectively. The study showed the presence of 16.574%, 15.943% and 10.168% alkaloids and 0.240%, 0.160% and 0.230% Q. alkaloid & N-oxides in leaves stem and fruit extracts respectively. 2.888%, 0.825% and 2.679% fats and waxes and 76.395%, 81.347% and 84.672% fibers were observed in leaves, stem and fruit extracts of this plant respectively