This study was aiming at investigating S. aureus in butter and ghee in Nyala city in South Darfour State during the year 2021. A total of 80 milk products' samples (40 butter and 40 ghee) were collected from 4 markets (Nyala, El Ban gadeed, Manawashy and El Laloba) in Nyala Locality. Samples were examined bacteriologically using conventional and rapid API methods. All butter samples (100%) collected from El Laloba and Manawashy markets were positive for S. aureus. Eighty percent of the samples collected from Nyala market were positive for S. aureus. Sixty percent of the samples collected from El Ban gadeed market were positive for S. aureus. All ghee samples (100%) collected from El Laloba and El Bangadeed markets were negative for S. aureus. Sixty percent of the samples collected from Nyala and Manawashy markets were positive for S. aureus. The prevalence of S. aureus in butter samples in Nyala Locality was 85%. The prevalence of S. aureus in butter samples in Nyala Locality was 30%. Butter in Nyala Locality is highly contaminated and ghee is less contaminated with S. aureus. Pasteurization and heat treatment are highly recommended before processing of milk.