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The phytochemical profiles and antioxidant activity of methanolic extract of Wheat Grains (WG) and 3 days old wheat seedlings (3dWS) were evaluated in the present study. Phytochemical analysis was performed for alkaloids, flavonoids, phenols, saponins, steroids, terpenoids, proteins and carbohydrates in WG and 3dWS extracts. Antioxidant activity was evaluated using DPPH and ABTS radical scavenging activity, total phenolic content and reducing power ability. In this study, the results of WG were compared with 3dWS. The results of phytochemical analysis showed the presence of all the above phytochemicals in the both 3dWS and WG extracts. When compared with WG, the 3dWS possesses high nutritional contents and antioxidant activity. Hence, it is suggested to add wheat seedling in the daily diet to reduce many diseases caused by antioxidant deficiency.

Ravikumar, et al.: Antioxidant Activity of Wheat SeedlingsThe present study was designed to evaluate the antioxidant activity of 5 organic solvent extracts (petroleum ether, n-hexane, chloroform, ethyl acetate and methanol) of wheat grains, 3, 5 and 7 days old wheat seedlings. To determine the antioxidant activity of five extracts of four different samples, 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, total phenolic content and ferrous reducing power ability were carried out. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging effect of chloroform and ethyl acetate extracts of 3 days old wheat seedlings was higher than wheat grains. Chloroform, ethyl acetate and methanol extracts of 3 days old wheat seedlings exhibited higher 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging effcet than extracts of other samples. The phenolic content was high in chloroform, ethyl acetate and methanol extract of 5 days old wheat seedlings. When compared with wheat grain, reducing power ability was high in chloroform, ethyl acetate and methanol extract of wheat seedlings, especially in 3 and 5 days old wheat seedlings. From the above results, it was concluded that chloroform, ethyl acetate and methanol extract of 3, 5 and 7 days old wheat seedlings showed better antioxidant activity than the wheat grain extracts. Hence, the results of the present study suggest the intake of wheat seedlings as a food supplement to combat the diseases caused by free radicals.

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