Materials and Methods Materials Serratiopeptidase was obtained from Advanced Enzyme Technologies, Thane, India. Eudragit L100 was gifted by Evonik Roehm Pharma Polymer, Mumbai, India. Clove oil, Tween 80, ethanol and HPMC K4M were purchased from S.D. Fine Chemicals, Mumbai, India. All other chemicals used were of analytical grades. Methods Preparation of buccal patch Non-emulsification method: Ten mg serratiopeptidase was dissolved in ethanol at ambient temperature under continuous stirring and then added to alcoholic HPMC solution. Later, clove oil and Eudragit L100 were added to form homogenous mixture. This mixture was poured in a petri dish and dried at room temperature for 12 h [7]. The dried film was removed and stored in a desiccator. Different patches of polymer concentrations of HPMC and Eudragit L100 were prepared in various ratios 1:1, 3:2, 2:3 and 4.5:5.5 were abbreviated as F1, F2, F3 and F4, respectively as shown in Table 1.

A simple, economical, selective, rapid and precise HPLC method has been developed for simultaneous estimation of ondansetron hydrochloride and famotidine in their bulk and pharmaceutical dosage forms. The study was carried out using a column of Kromasil 100-C8 (250mm×4.6mm, 5µm) with a mobile phase consisting degassed mixture of phosphate buffer and methanol (50:50) at a flow rate of 0.6ml/min. The retention time of ondansetron hydrochloride and famotidine at wavelength 250 nm was found to be 7.74 and 4.44min, respectively. This developed method was also validated for linearity, accuracy, system suitability and robustness. Keywords:

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MethodsSolvent dispersion: An equivalent quantity of β-cyclodextrin and ammonia, were heated in a water bath at 100°C. Eventually the indomethacin was added to the complex and kept under AbstractThe objective of present work was to compare the effect of different solubility enhancement methods like solvent dispersion, physical mixture, hydrotropy, solid dispersion and freeze drying on in-vitro release and stability studies of indomethacin. Indomethacin, BCS class II NSAIDs, acts by inhibiting COX-I and II enzymes and uses in treating various conditions like rheumatoid arthritis, pain and headache. Inclusion complex formulations were prepared using different methods like solvent dispersion, physical mixing, hydrotropy, solid dispersion and freeze drying. Particle size and zeta potential observed varied range of values between 479.4±1.12 to 1417±2.37nm and in between -15.3±1.08 to -24.9±1.18mV respectively. The highest solubility was observed in solid dispersion due to highest reduction of particle size. In-vitro drug release study showed controlled indomethacin release pattern in solid dispersion for 24 h as compared to other inclusion complexes. Solid dispersion method emphasizes its importance in enhancing the solubility and bioavailability, controlled release and stability of indomethacin.