Energy consumption has inflated steadily over the last century because the world population has fully grown and additional countries became industrialised. Bioethanol is an alcohol produced by fermentation of plant biomass, containing carbohydrate and its production depends upon feedstock availability, variability, and sustainability. The selection of feedstock and its pretreatment is an important part of bioethanol production process. In present work, the exploration of the potential of agro-waste rice residues such as, rice bran and rice husk was done, because it contains sufficient amount of carbohydrate which can be ferment into bioethanol. The aim of the research was also to investigate how different pretreatment methods with moderate conditions differ in hydrolysis and fermentation efficiencies. Pretreatment plays an important role in the hydrolysis of cellulose and lignocellulose. It was found that biological pretreatment was a most effective method in terms of production of bioethanol and it enhances the production as well as fermentation efficiency.