Background: Chronic kidney disease (CKD) interacts with thyroid disease and cardiovascular disease (CVD). Our research aimed to analyze the correlation between echocardiographic parameters E / A, E / E ', E' / A ', LVEF and thyroid autoantibodies, and evaluate the role of thyroid autoimmunity in the development of CVD in patients with stages 3-5 CKD.Methods: The patients who were diagnosed as stages 3-5 CKD in our department from January 2015 to May 2019 were recruited. We collected the routine medical history, general clinical data, and laboratory test index of patients. Echocardiography is performed by a trained echocardiographer to measure mitral valve blood flow velocity (E) in early diastole and Mitral valve flow velocity (A), E / A ratio, mitral annulus velocity (E ') in early diastole, mitral annulus velocity (A') in end-diastole, E / E 'ratio, and E' / A ' ratio. The SPSS 22.0 statistical software was used to analyze the data.Results: A total of 1164 patients with stages 3-5 CKD were included. Thyroglobulin antibody (TGAb) was negatively correlated with eGFR (r = -0.287, P <0.05). Thyrotropin receptor antibody (TRAb) was significantly positively correlated with CRP (r = 0.206, P <0.001). The titers of TPOAb and TGAb in male diabetic patients were higher (r = 0.137, P = 0.023; r = 0.159, P = 0.011). In female patients, both TPOAb and TGAb are significantly negatively correlated with HGB (r = -0.213, P = 0.018; r = -0.188, P = 0.019). The E / E’ of patients with TPOAb positive was higher (r = 0.181, P ＜ 0.001). The LVEF in patients with TPOAb positive were higher (r = 0.159, P = 0.007). In addition, LVEF was significantly negatively correlated with TRAb (r = -0.112, P = 0.026).Conclusion: The prevalence of AITD in stages 3-5 CKD gradually increases with the decline of renal function, and the titers of TPOAb and TGAb also gradually increase. In patients with stages 3-5 CKD, AITD may accelerate the incidence of CVD in CKD patients by affecting TG levels, accelerating the occurrence of anemia, and promoting the micro-inflammation. Female patients with high titers of TPOAb and TGAb should be paid more attention. The average E/E' of patients with stage 5 CKD was 16. Women with low FT3 and TPOAb positive maybe more likely to develop diastolic heart failure.