BACKGROUND: Laptop computers are used in various places and situations. The number of laptop users experiencing musculoskeletal disorders (MSDs) has increased drastically due to, in part, inappropriate workstations. OBJECTIVE: To investigate the neck and shoulder postures, and muscle activity relative to perceived pain when using the laptop at a low-height table, sofa, and bed. METHODS: Twenty male participants aged 18-25 years were randomly assigned to perform laptop computer operation at 3 workstations for 10 minutes during which neck and shoulder angles, muscle activity, and pain were recorded by using an Electrogoniometer, Electromyography (EMG), and visual analog scale (VAS), respectively. RESULTS: Neck flexions when working at the sofa (18.6 • ± 12.2 • ) and bed (17.2 • ± 10.5 • ) were significantly (p < 0.05) greater than that at the low-height table (7.8 • ± 6.5 • ). However, shoulder flexion when working at the low-height table (28.2 • ± 13.0 • ) was significantly (p < 0.05) greater than that at the sofa (13.8 • ± 8.6 • ) and bed (10.91 • ± 7.8 • ). Working at the low-height table caused the shoulder flexor muscle activity to be significantly (p < 0.05) higher than working at the sofa and bed. Neck pain was reported during laptop computer use at the sofa and bed, and upper back pain when working at the low-height table. CONCLUSIONS: High neck flexion and pain were found while working at sofa and bed, whereas high muscle activity at shoulder and upper back pain were found while working at the low-height table.