This study examined how the attitudes of conservative American Protestants attending Midwestern churches might vary as a function of religious beliefs, gender, and exposure to scenarios of people from stigmatized groups who were asking for spiritual care. Results of the 2 (scenario gender) x 2 (scenario sexual orientation) x 2 (scenario mental health issue) x 2 (participant gender) MANOVA revealed significance for sexual orientation bias (Λ = .79, F (2, 88) = 11.94, p < .001, η2 = .21) and participant gender bias (Λ = .91, F (2, 88) = 4.13, p = .02, η2 = .09. Follow-up ANOVA's revealed different effects depending on whether participants reported personal or perceived group attitudes. We discuss the results in terms of social comparison theory and projection.