In addition, neither 3',5'-AMP nor ACTH stimulated the transformation of [7a-3H]pregnenolone (3/3-hydroxy-5-pregnen-3-one) or [7-3H]progesterone (4-pregnene-3,20-dione) to corticosteroids. The results indicate that both 3',5'-AMP and ACTH stimulate at the same site in the biosynthetic pathway for corticosteroids, i.e., between cholesterol and pregnenolone, and suggest that 3 ',5 '-AMP is an obligatory intermediate in the action of ACTH.
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