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Examination of numerous sera from patients with infectious mononucleodis ( I M ) , Burkitt 's lymphoma ( B L ) or nasopharyngeal carcinoma ( N P C ) for antibodieh to Epstein-Barr virus ( E B V ) induced early antigens ( E A ) revealed two distinct patterns of immunofluorescence in abortively E B V-infected Raji cells. One showed diguse ( D ) staining of the nucleus and cytoplasm of invaded cells, the other ( R ) was restricted to masses in the cytoplasm. Although D-or R-reactive Raji cells became detectable at similar times after exposure to EBV, the percentages of D-positive cells initially exceeded R-positive cells but ultimately both were nearly equal in number. R-positive cells almost invariably contained also D. In EBV-exposed R P M l 64-10 cells, frequently only D was synthesized. D antigen, in contrast to R , resisted fixation by methanol or cthanol, whereas R proved more resistant than D to proteolytic enzymes. Comparative serum titration on acetone, respectively ethanol-fixed Ruji-EBV cell smears revealed that the transitory anti-EA response observed in many I

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