This paper is a case study that sets out to explore the extent to which the local residents of the region of Northern Karpathos and Saria in the southeast corner of the Aegean have positive attitudes and perceptions towards ecotourism ventures for sustainable development. The area is included in the European Ecological Network Natura 2000 and was chosen for studying since it constitutes an ideal location for the promotion of soft and alternative forms of tourism. The investigation of the attitudes of the inhabitants of the Community of Olympos, in Northern Karpathos was carried out using as research tool a questionnaire that was drawn up specifically for the needs of the present work. The attempt to provide a systematic way of studying the inhabitants' attitudes led to the proposition of a framework that contains the necessary conditions for sustainable ecotourism development. This is based on three fundamental factors, namely: (a) environmental conservation, (b) environmental education and (c) empowerment of local community. The main conclusion drawn was that the perceptions of the local people accord with the basic principles of ecotourism. Residents (a) showed a high degree of awareness and sensitivity regarding environmental conservation issues in the area, (b) recognised the need for environmental education concerning ecotourism activities and (c) had a favourable attitude towards the implementation of initiatives in the ecotourism field aimed at their empowerment and at promoting sustainable development in the area. The participants' place of permanent residence and level of education appeared to influence significantly their perceptions of this alternative form of tourism.