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An assay procedure for the quantitative determination of nicarbazin, which is an equimolar complex of 4,4'-dinitrocarbanilide (DNC) and 2-hydroxy-4,6-dimethylpyrimidine (HDP), in chicken muscle, liver, kidney, and skin-fat is described. The polarographically active moiety DNC is extracted from the tissue by solvent extraction and is determined quantitatively by differential pulse polarography. The estimated sensitivity of the

A procedure has been developed for the isolation and gas chromatographic determination of the coccidiostat pyrimethamine (2,4-diamino-5-p-chlorophenyl-6-ethylpyrimidine) in tissue.

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