Objective: Our aim was to assess the prevalence and predictors of well-being among Brazilian industry workers on returning to the physical workplace during the COVID-19 pandemic. Methods: Two thousand two hundred forty-one participants completed an online survey between October and November 2021. Well-being was assessed with the World Health Organization Well-being Index, and the questionnaire also addressed sociodemographic and occupational characteristics and the health of workers and their contacts. Associations were estimated with logistic regression models. Results: The prevalence of adequate well-being was 63.15%. The predictors of poor well-being included being female, younger, working exclusively from home or on a hybrid model, having comorbid disorders, and living with someone with any comorbidity. Conclusions: The identification of vulnerable groups with poor well-being may help organizations to direct efforts to the unmet psychological needs of these employees and develop well-being programs during this transition.