“Titanium dioxide (TiO 2 ) is a semiconductor material that exhibits antibacterial activity due to its photocatalytic properties under ultraviolet light. On the other hand, silver also exhibits strong antibacterial activity towards a wide range of microorganisms and TiO 2 with silver addition exhibits more efficient photocatalytic properties than unmodified TiO 2 . In this work, TiO 2 nanoparticles were synthesized by the hydrothermal method and modified with silver by two different methods: wet impregnation ( Ex situ ) and In situ incorporation. The antimicrobial activity of TiO 2 nanoparticles synthesized and modified by both methods was evaluated against Escherichia coli and Staphylococcus aureus . The results showed that TiO 2 nanoparticles have anatase phase. Also, spherical morphology with a mean particle size around 10.6 nm was obtained. The presence of silver in the modified TiO 2 nanoparticles was confirmed by EDS and XPS. TiO 2 particles modified by the Ex situ method, showed a better bactericidal activity compared to the particles modified by In situ incorporation method and TiO 2 unmodified nanoparticles. This study demonstrated that both methods used to modify the titanium dioxide nanoparticles are effective as bactericidal materials and better results were found for the Ex situ method.”

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