The Bourbon coffee cultivar has a high potential for producing a quality beverage, which is highly valued in specialty coffee markets. The objective of this study was to verify the occurrence of more promising genotypes for the production of specialty coffees in three different environments. In addition, the chemical composition of the grains was studied with a view toward relating these compounds to the genotypes and environments and to the interaction of these factors.The experimental design included three Brazilian locations in combination with different Arabica coffees, including eleven bourbon and three commercial genotypes. Trigonelline, caffeine and 5-caffeoylquinic acid (5-CQA) from coffee bean samples were analyzed by HPLC, and sensory analyses were performed by professional cuppers accredited by the Coffee Quality Institute. A group of Bourbon genotypes with potential for the production of specialty coffees was identified for each environment assessed. The trigonelline content allowed for discrimination among the three environments studied.
O objetivo deste trabalho foi avaliar os perfis proteicos e o desempenho fisiológico de sementes de café submetidas a diferentes métodos de processamento e secagem. Foram avaliados os processamentos por via seca e úmida, e as secagens natural, em terreiro, e artificial a 60ºC, ou a 60ºC até 30% de umidade e 40ºC até teor final de 11% (base úmida). Após serem processadas e secadas, as sementes foram avaliadas quanto ao desempenho fisiológico e submetidas a análises bioquímicas, por meio da eletroforese de proteínas resistentes ao calor LEA ("late embryogenesis abundant") e das enzimas superóxido dismutase, catalase, peroxidase, esterase, polifenoloxidase, isocitrato desidrogenase, álcool desidrogenase e malato desidrogenase. O perfil proteico de sementes de café é afetado pelo método de processamento e de secagem. Os cafés processados por via úmida apresentam maior tolerância à secagem - revelada pela maior atividade de enzimas antioxidativas e pelo melhor desempenho fisiológico - do que os processados por via seca. A atividade de proteínas resistentes ao calor e de enzimas antioxidantes é variável promissora para diferenciar a qualidade dos cafés submetidos a diferentes manejos pós-colheita.
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