Attributed to their unique properties zinc oxide nanoparticles (ZnONPs) in recent years, have received considerable attention. Notably, research has demonstrated that zinc in living organisms is an essential nutrient. For example, both eukaryotes and prokaryotes including fungi, bacteria, and yeast by using protein, enzyme or microbial cells and other biomolecules compounds are exploited for the synthesis of ZnONPs in either an extracellular or intracellular route. However, the properties of nanoparticles (NPs) are depended upon their shape and size. ZnONPs demonstrated antimicrobial properties, making them unique for different applications. Therefore, in the current study Due to its extensive antimicrobial activity, eco-friendly and simplicity, ZnO Nanoparticles (ZnONPs) synthesis has gained much attention. So, this review was done to concentrate about the green synthesized of ZnONPs by bacteria and their application. Also, the study of their effect as biopesticides against the House Fly, Musca domestica L was mentioned.