Novel Zn(II) complexes with the general formula: [Zn(furo) 2 (L) n ], n = 1 or 2, (furo = furosemide = (4-chloro-2-(furan-2-ylmethylamino)-5-sulfamoylbenzoic acid) were prepared. The complexes [Zn(furo) 2 (MeOH) 2 ] (1; MeOH = methanol), [Zn(furo) 2 (2-ampy) 2 ] (2; 2-ampy = 2-aminopyridine), [Zn(furo) 2 (2-ammepy) 2 ] (3; 2-ammepy = 2-aminomethylpyridine), [Zn(furo) 2 (H 2 O)(2,2-bipy)] (4; 2,2′bipy = 2,2′-bipyridine), [Zn(furo) 2 (H 2 O)(4,4′-bipy)] (5; 4,4′-bipy = 4,4′bipyridine), [Zn(furo) 2 (1,10-phen)] (6; 1,10-phen = 1,10-phenanthroline),[Zn(furo) 2 (2,9-dmp)] (7; 2,9-dmp = 2,9-dimethyl-1,10-phenanthroline), and [Zn (furo) 2 (quin) 2 ] (8; quin = quinoline) were synthesized and characterized using different techniques such as IR, UV-Vis, 1 H NMR, 13 C NMR, LC/MS and others. The crystal structure of complex (4) was determined using singlecrystal X-ray diffraction.The anti-bacterial activity of complexes (1-8) was tested using agar diffusion method against three gram-positive (Staphylococcus aureus, Bacillus subtilis and Staphylococcus epidermidis) and three gram-negative bacteria (Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa). The obtained results showed different Inhibition Zone Diameters (IZD) with various anti-bacterial activities against the selected gram-positive and gram-negative bacteria. In addition, the rate of bis-(4-nitrophenyl) phosphate hydrolysis was measured at different temperatures, different pH values and different concentrations. The rates for the eight complexes were in the following order: complex 4 > 2 > 5 > 8 > 7 > 6 > 3 > 1. KEYWORDS anti-bacterial activity, bioactive nitrogen based ligands, bis-(4-nitrophenyl) phosphate, furosemide, zinc(II) complexes