The current study aimed to reveal the impact of the Arab Spring on the concept of political participation among Jordanian university students (Isra University as a model), with different variables (gender, age, and school year), the researchers used the descriptive analytical approach, as a questionnaire was developed for this purpose after reviewing the theoretical literature in this field, and it was applied on a sample of (192) male and female students from Al-Isra University in the second semester of the academic year 2019. By using appropriate statistical analyzes, the results of the study revealed that the effect of the Arab Spring on the concept of political participation among Jordanian university students came with an average degree, as the results showed that there were no statistically significant differences for the impact of the Arab Spring on the concept of political participation among Jordanian university students due to gender and age variables, while a statistically significant difference was observed between the arithmetic averages of the effect of the Arab Spring on the concept of political participation among Jordanian university students due to the variable of the school year in favor of the first, second and third years when compared to the fourth and fifth years.