Purpose This paper aims to measure the perceptions of librarians about information literacy (IL) instruction, their current IL practices and the problems they face while offering IL instructional programs in public and private sector university libraries in Islamabad, the capital city of Pakistan. Design/methodology/approach A quantitative research design was adopted, and a survey was carried out for this study to collect the data through an online questionnaire for the university librarians. All librarians (a total of 120) were approached to collect the data, of whom 92 (76.6%) responded to the survey. Findings The findings reveal that the librarians working in the university libraries of Islamabad are aware of the significance of IL instruction to fulfil the information needs of their library users. A majority of the librarians indicated having adequate skills to offer IL instruction. Moreover, the majority of the librarians are offering IL instruction to users at their respective universities. However, some of the barriers affecting IL instruction programs were also reported including the absence of IL instruction courses in the library and information science (LIS) curriculum, a shortage of trained library professional staff to provide IL instruction, a lack of commitment among librarians for IL instruction, a lack of training opportunities for librarians and a lack of interest from library users. These obstacles have a negative effect on IL instruction in the university libraries of Islamabad. Therefore, this study suggests that librarians are needed to take several necessary steps to overcome these obstacles to achieve the best results in IL instruction programs. Originality/value This study presents a picture of the status of IL skills, current practices and barriers to offering IL instruction in the university libraries of Islamabad. This research bridges the gap in the existing literature and will help to create awareness about the importance of IL skills among LIS professionals in Pakistan. Moreover, the findings of this study will help encourage librarians for the enhancement of IL instruction services in university libraries of Islamabad in particular and Pakistan in general.