This study was aimed to isolate and identify the fungus Trichophyton violaceum from human skin specimens and evaluate the activity of antibacterial and plant essential oils. The results showed of direct microscopy by using KOH examination and morphological identification, nine samples from skin were positive in KOH test. Microscopical examination appeared that colonies of T. violaceum were coarse with abundant aerial mycelium on SDA, growth rates 6-8cm/14 days. The color changes with age from white in the middle to brownish yellow at the edges which was more intense on the reverse side. Susceptibility test to antibacterial showed that 100% of T.Violaceum T2 isolate was resistant to TE (Tetracycline), AK (Amikacin), CTX (Cefotaxime), CFM (Cefixime), CIP (Ciprofloxacin), ERY (Erythromycin) and STR (Streptomycin). Also results showed that all plant essential oils at different concentrations significantly inhibit growth of T. violaceum T2. However, the peppermint oil, Myrrh oil, Cardamom oil, Chamomile oil and Castor oil at highest concentration (25mg / ml) caused highest reduction of mycelia growth (100%) followed by Olive oil (67.3%) and Clove oil (64%) at the same concentration, compared with the control treatment.
The aim of the study was to use lactic acid bacteria (LAB), as probiotic, to treat growth and adhesion property of Proteus mirabilis isolated from patients suffering from urinary tract infection (UTI). For this purpose, one P. mirabilis isolate (P.M.9) was selected out of 9 isolates obtained from 150 urine specimens. Due to its resistance to 11 antibiotics tested, this isolate was treated with three-fold concentrated filtrates of two lactobacillus isolates (as probiotic). Results after treatment, showed that the filtrates exhibited significant inhibitory effect against the pathogenic P.M.9 and its adhesion property especially when only an average of 3-10 bacteria /cell were adhered to each epithelial cell compared to 44-55 bacteria/cell.
In this work, sample of workers who are in contact with heavy metals were selected. Those were distributed as follow car customizers and welders (CW) (25), bakers (20) local power generator operators "operators" (15), and control men who their occupation was far from such contact (20). The study included measurement of testosterone, LH and FSH as fertility hormones, measurement of lead and cadmium (Cd) as heavy metals, and exposure to heat during the working day. Results showed that fertility hormones were within the control levels in all subjects; only a significant increase (p≤0.05) was recorded in LH in (CW) compared with control. Seminal fluid analysis (SFA), showed a decrease in total sperm count in all samples when compared with control. No significant raise was found in heavy metals in bakers, while a significant elevation of both types of heavy metals were recorded in blood of (CW) and (operators). It is concluded that occupation might result in elevating levels of heavy metals in welders and car customizers, and local power generator operators.
This study was designed to evaluate the effects of cinnamon and probiotics both alone and in combination against pathogenic bacteria isolated from UTI patients. One hundred and ten male and female patients with a urinary tract infection (UTI) who visited private health care facilities were asked to provide urine samples. The urine samples were inoculated on Blood and MacConkey agars and incubated for 24 hrs. at 37 degrees Celsius. After 24 hours of incubation, no development was seen in the cultures, thus they were kept in the incubator for a further 48 hours. Gram staining was used to classify bacteria, and then conventional microbiological culture and biochemical properties were used to identify the isolates. Cinnamomum bark oil was extracted manually. Mueller Hinton (MH) agar plates were infected with 108 CFU/ml suspensions of microorganisms; this method was used to determine the agar disc diffusion test. Discs of sterile Whatman paper No. 3 impregnated with 10 g of the compounds were placed on top of MH agar that had been infected with bacteria. For 18 hours, the plates were kept at 37°C. Inhibition zones against the various bacterial strains were used to evaluate the antibacterial impact.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.