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Root P. speciosa † Instability index < 40 and > 40 is predicted the protein as stable and unstable, respectively. ‡ Aliphatic index (alanine, valine, isoleucine, and leucine) is regarded as a positive factor for the increase of thermostability of globular proteins.$ GRAVY is calculated as the sum of hydropathy values of all the amino acids, divided by the number of residues in the sequence. Negative and positive GRAVY values indicate that the protein is non-polar and polar, respectively.

To identify the genetic diversity of wild edible Termitomyces heimii (T. heimii) mushrooms, they were collected from three geographical locations in Baleswar, Kendujhar and Mayurbhanj districts in the North Odisha of India and were assessed using RAPD markers. 5 primers produced 43 scorable bands with 100% of the polymorphism. Polymorphic banding patterns with the number of amplified fragments varied from 6 (OPT 5 and OPT 12) to 11 (OPT 1) with an average of 8.6 per primer. The PIC values ranged from minimum value of 0.30 to maximum of 0.44 with an average value of 0.38. The heterozygosity (H) varied from 0.29 to 0.43 with an average of 0.36. The number of effective alleles (Ne), Shannon’s information index (I) and Nei’s gene diversity (h) were observed at population level as 1.604, 0.542 and 0.361 respectively whereas at species level the values were observed as 1.675, 0.684 and 0.459 respectively. These results showed the abundant genetic variability in the wild T. heimii. Nei’s gene diversity investigation showed that the genetic diversity was mainly found within geographical populations. The results of Mantel tests, UPGMA analysis, PCoA and PCA showed that geographical isolation is an important factor for the observed genetic differentiation.