Seven-transmembrane receptors (7TMRs) signal through the well described heterotrimeric G proteins but can also activate G protein-independent signaling pathways of which the impact and complexity are less understood. The angiotensin II type 1 receptor (AT 1 R) is a prototypical 7TMR and an important drug target in cardiovascular diseases. "Biased agonists" with intrinsic "functional selectivity" that simultaneously blocks G␣ q protein activity and activates G protein-independent pathways of the AT 1 R confer important perspectives in treatment of cardiovascular diseases. In this study, we performed a global quantitative phosphoproteomics analysis of the AT 1 R signaling network. We analyzed ligand-stimulated SILAC (stable isotope labeling by amino acids in cell culture) cells by high resolution (LTQ-Orbitrap) MS and compared the phosphoproteomes of the AT 1 R agonist angiotensin II and the biased agonist [Sar 1 ,Ile 4 ,Ile 8 ]angiotensin II (SII angiotensin II), which only activates the G␣ q protein-independent signaling. We quantified more than 10,000 phosphorylation sites of which 1183 were regulated by angiotensin II or its analogue SII angiotensin II. 36% of the AT 1 R-regulated phosphorylations were regulated by SII angiotensin II. Analysis of phosphorylation site patterns showed a striking distinction between protein kinases activated by G␣ q protein-dependent and -independent mechanisms, and we now place protein kinase D as a key protein involved in both G␣ q -dependent and -independent AT 1 R signaling. This study provides substantial novel insight into angiotensin II signal transduction and is the first study dissecting the differences between a full agonist and a biased agonist from a 7TMR on a systems-wide scale. Importantly, it reveals a previously unappreciated diversity and quantity of G␣ q protein-independent signaling and uncovers novel signaling pathways. We foresee that the amount and diversity of G protein-independent signaling may be more pronounced than previously recognized for other 7TMRs as well. Quantitative mass spectrometry is a promising tool for evaluation of the signaling properties of biased agonists to other receptors in the future. Molecular & Cellular Proteomics 9:1540 -1553, 2010. Seven-transmembrane receptors (7TMRs)1 constitute the largest family of plasma membrane receptors. These receptors regulate a variety of biological processes and are currently the most prominent therapeutic targets, highlighted by an abundance of clinically available drugs (1-5). Their cellular responses were until recently thought to depend primarily on G protein activation and generation of second messengers such as inositol 1,4,5-trisphosphate, diacylglycerol, and cAMP, reflected by the term G protein-coupled receptors. However, the 7TMR structure also confers activation of G protein-independent signaling initiated by direct interaction with ␤-arrestin or tyrosine kinases (1-3, 6, 7). The discovery of alternative signaling pathways to the G protein-initiated pathways has also led to the discovery o...