No abstract

Purpose: To evaluate the in vitro e cacy of cidofovir, ganciclovir, povidone iodine, chlorhexidine, and cyclosporine A on adenovirus genotype 8 Methods: Conjunctival samples were collected from patients with adenoviral conjunctivitis and cultured in A549 cells. Adenovirus diagnosis was con rmed by RT-PCR. For each drug, the 50% cytotoxic concentration (CC ) was determined. Subsequently, the antiviral activity was tested at concentrations below CC , and the 50% inhibitor concentration (IC ) of drugs was determined.Results: While the IC of cidofovir against adenovirus genotype 8 was 3.07 ± 0.8 µM, ganciclovir, povidone iodine, chlorhexidine, and cyclosporine A were not found to be effective against adenovirus genotype 8 at concentrations below the CC value.Conclusions: Cidofovir was found effective and the IC value was within the ranges in the literature. Ganciclovir and cyclosporine A were found to be ineffective at doses below the cytotoxic dose, povidone iodine and chlorhexidine were found to be highly cytotoxic.

Giriş: Human papillomavirüs (HPV) servikal kanserin primer sebebidir. HPV ile birlikte Chlamydia trachomatis servikal intraepitelyal neoplazi gelişimi için potansiyel bir kofaktör olarak rol oynamaktadır. Ürogenital bölgede kommensal olarak bulunan Ureaplasma urealyticum'un ise ürogenital infeksiyonlara katkıda bulunduğu kabul edilmiştir. Bu çalışmanın amacı HPV pozitif ve HPV negatif kadınlarda C. trachomatis ve U. urealyticum koinfeksiyonlarının araştırılmasıdır.

Aim: BK virus (BKV)-associated hemorrhagic cystitis (HC) is a common complication in patients after hematopoietic stem cell transplantation (HSCT). The aim of this study was to investigate the incidence of BKV infection in pediatric patients receiving HSCT. Material and Methods: Total of 51 patients aged between 16 months and 16 years old and followed up between October 2015 and September 2017 were included in the study. The patients were monitored by quantitative real-time polymerase chain reaction (Anatolia Geneworks, Turkey) test for the detection of BKV DNA in urine and blood. Results: Of patients, 46 received allogeneic HSCT and 5 autologous HSCT. BKV DNA positivity was detected in urine and/or blood of total 27 (52.9%) patients in whom 26 (56.5%) of 46 patients with allogeneic transplantation, and 1 (20.0%) of 5 patients with autologous transplantation. BKV viral load in urine >10 7 copies/ml required for preemptive treatment was detected in 12 (26.1%) of 46 patients received allogeneic HSCT. The development of HC was prevented in 9 (75.0%) of the 12 patients given preemptive treatment, while 3 (25.0%) cases developed HC and cured by treatment. BKV viruria was detected >10 9 copies/ml in two weeks before the onset of HC and was accepted as a prognostic indicator for predictive diagnosis of HC. BKV viremia was found >10 4 copies/ml in 1 patient within two weeks before the onset of cystitis. Conclusion:Screening for BKV infection, especially BKV viruria in HSCT patients, is recommended for the predictive diagnosis of HC in patients at high risk.

ÖZET Giriş: Hematopoietik kök hücre transplantasyonu (HKHT) yapılan hastalarda immünsüpresif tedaviye bağlı viral infeksiyonlar özellikle sitomegalovirüs (CMV), Epstein-Barr virüs (EBV) ve adenovirüs (ADV) infeksiyonları morbidite ve mortalitenin başlıca sebebidir. Bu çalış-mada, HKHT yapılan çocuk hastalarda CMV, EBV ve ADV infeksiyonlarının insidansının araştırılması amaçlanmıştır. Materyal ve