Occurrence, ecology and phylogeny of banana streak badnavirus (BSV) and cucumber mosaic cucumovirus (CMV) in Musa sp. production areas of the Mediterranean coastline of Turkey -5935 -

Alfalfa Mosaic Virus) ve ZYMV (Zucchini Yellow Mosaic Virus) gibi bilinen süs bitkisi virüslerine karşı testlenmiştir. DAS-ELISA'da CMV pozitif örnek (8 adet, %13.1) sonuçları, 100 mg yapraktan ekstrakte edilen toplam RNA'ların RT-PCR'da kullanılmasıyla doğrulanmıştır. Sense CMVr ve Anti sense CMVf primerleriyle yapılan RT-PCR sonucu, 513 bp cDNA bandları %1.5'lik agaroz jelde gözlenmiştir. Antalya'da her dem yeşil olan Sütotu'nda CMV'nin serolojik ve moleküler tekniklerle ilk kaydı olan bu çalışma belki de türler ve bölgeler arasında kontrolsüz yayılımının kanıtıdır.

Surveys were conducted at tomato production sites in Adana province in 2019. 53 tomato plants have been collected showing the virus symptom due to determining its prevalence. The collected samples were tested for 13 different viral agents harmful to tomatoes using ELISA and RT-PCR methods. 24.52% TYLCV and 9.43% PLRV were detected from the tested samples. When DNA sequencing comparisons are made from RT-PCR products, 98,74% of PLRV isolates in tomato samples collected from Adana province are homogical similarity with Belgium potato isolate (KX364206.1). Also it clustered at 99.37% similar to same branch with New Zealand (GU002341.1) (BLAST at NCBI).

Observations were carried out on Anthurium sp growing at Mediterranean region (Adana, Mersin and Antalya provinces) of Turkey in 2015-2017. During the studies virus-like symptoms in plants; systemic chlorotic mosaic and irregular uniformity on leaf and petals were recognized. Eleven of 73 samples showed positive response with the Potyvirus group specific antibodies and their results were also confirmed by RT-PCR. The 327 bp products via using the Potyvirus-specific primers, MJ1 and MJ2 were run in 1.5% agarose gel. Further amplifications were made with the 3'UTR and 3'end of the coat protein of the viral genome. RT-PCR analysis using the primer MJ1 and M4T resulted in an amplification of 719 bp amplicon. Direct sequencing of RT-PCR products and BLAST results have showed that there were two clades between 96-99% among the Mediterranean coast DsMV isolates. The data are available in Genebank with accession Number MK758088/Antalya; MK758087/Yenice). DsMV infection on Anthurium sp, was determined serologically and molecularly. To our knowledge, this is the first molecular record describing the occurrence, distribution and genome organisation of DsMV isolates that have been infecting Flamingo flowers at the Mediterranean coast of Turkey. Findings may have shown an evidence about uncontrolled distribution from Northern corner to Southern side.