Shiga-like toxin producing Escherichia coli is still an important public issue which causes extremely dangerous health problems. This study was planned in order to examine the inhibitory effect of Modified Atmosphere Packaging application on E. coli O157 and O157: H7. The purposes of the present study were to detect E. coli O157 and O157: H7 strains from ground and cubed beef. A total of 100 MAP cattle meat products (50 minced meat, 50 meat cubes) were collected from the markets and butchers in Samsun province between May and October 2013. According to results, 1(1/50-2%) E. coli O157 and 1(1/50-2%) E. coli O157: H7 strains isolated from 50 ground beef samples, while 1 (1/50-2%) E. coli O157 strain was identified from 50 cubed beef samples. It was determined that E. coli O157 isolate obtained from the MAP ground beef carried stx 1, stx 2 genes; E. coli O157: H7 isolate carried stx 1, stx 2, eaeA and hylA genes while E. coli O157 isolate obtained from the MAP cubed meat only carried the stx 2 gene. In antibiogram test, both E. coli O157 isolates were resistant to streptomycin and one E. coli O157: H7 isolate was resistant to streptomycin, cephalothin and tetracycline. As a consequence; in order to protect public health, products should be kept in proper hygienic and technical conditions during sale and storage and use of uncontrolled antibiotics should be avoided. H7 ile, 50 adet MAP sığır kuşbaşı örneğinin 1'inin (1/50-%2) ise E. coli O157 ile kontamine olduğu belirlenmiştir. Yapılan genotiplendirme sonucunda MAP sığır kıyma örneklerinden elde edilen E. coli O157 izolatının stx1, stx2, E. coli O157: H7 izolatının ise stx1, stx2, eaeA ve hlyA gen bölgelerini taşıdığı, MAP sığır kuşbaşı örneklerinden elde edilen E. coli O157 izolatının ise sadece stx2 gen bölgesini taşıdığı belirlenmiştir. Fenotipik antibiyotik profil analizinde E. coli O157 izolatlarının her ikisinin yalnızca streptomisine karşı dirençli olduğu bununla beraber E. coli O157: H7 izolatının ise streptomisin, sefalothin ve tetrasiklin antibiyotiklerine karşı dirençli olduğu saptanmıştır. Sonuç olarak halk sağlığının korunması amacıyla ürünlerin uygun teknik ve hijyenik şartlarda satışa sunulması ve muhafaza edilmesine dikkat edilmesi, ayrıca izolatların antibiyotiklere direnç göstermesi nedeniyle kontrolsüz antibiyotik kullanımının önlenmesi önerilmektedir.
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