Global warming due to the emissio n of carbon dioxide (CO 2 ) gas, by the anthropogenic sources is a great threat to the environment. A part of this gas is absorbed by so il bacteria as well as aquatic bacteria and it is converted into inso luble calcium carbonate (CaCO 3 ) or calcite. Increased calcite concentration in water and agricultural land creates many problems to the human. In this invest igation, an attempt has been made to carry out some experiments to iso late some bacteria from cow dung, which have both calcite so lubilizat ion and urease activit ies. Isolated bacteria so lubilize calcite due to the secretion of citric acid, oxalic acid and sanazine pigment. All bacterial iso lates were identified by 16S rRNA gene sequencing and phylogenet ic relat ionship among them was also studied by MEGA 6 software.

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Experimental studies has been carried out to isolate and identify an active antifilarial compound from Vitex negundo L. plant as it has been used for treatment against filariasis in Indian traditional system of medicine. In vitro antifilarial assay has been carried out against adult filarial parasite Setaria cervi worms by both worm motility and MTT reduction assays. Levels of oxidative stress parameters MDA, carbonyl content and nitric oxide levels have been detected. The isolated compound exhibited significant antifilarial activity in dose dependent manner. The active compound has been chemically characterized and identified as 4,5-diethyl-3′-ethoxy-pyro-flavone.