The increasing number of stray dogs and the lack of sufficient data on the prevalence of leptospirosis among dogs were the main reasons for conducting this research in different populations of dogs on the territory of Bosnia and Herzegovina. A total of 300 serum samples were tested from three different categories of dogs of various breeds from 12 cities. Twelve leptospiral serovars were used in the microscopic agglutination test (MAT). The presence of specific antibodies was confirmed for eight serovars. The proportion of seropositive dogs was 22.3% (67/300). The highest seropositivity (n = 38; 42.7%) was found for the serovar Pomona. The seropositivity rates found for the other serovars tested were as follow: Canicola (14.6%), Icterohaemorrhagiae (13.5%), Sejroe (12.4%), Autumnalis (12.4%), Grippotyphosa (2.2%), Bratislava (1.1%), and Australis (1.1%). The highest number of positive responses was obtained at the serum dilution of 1:100 (39.3%, n = 35). The highest number of positive reactions was identified in the category of 'house dogs' (29.3%, 29/99) followed by 'stray dogs' (21.6%, 24/111), while the lowest number of positive tests was recorded in the category of 'guard/ hunting dogs' (15.6%, 14/90). Vaccination with tetravalent vaccines, including the serovars Icterohaemorrhagiae, Canicola, Pomona and Grippotyphosa could be an effective measure for the prevention of canine leptospirosis.

After cannibalism had appeared in the reproductive units of a white mouse colony, treatment against confirmed Hymenolepis nana, a tapeworm with zoonotic potential, was performed on 67 mice in the reproductive and nursery units. Faecal droppings were evaluated by flotation and sedimentation methods. The sedimentation method revealed a higher number of positive results before, during and after the treatment, but the flotation method yielded some additional positive cases. In the reproductive unit, H. nana eggs were confirmed in 50% of the tested mice by the flotation and in 70% by the sedimentation method. In the nursery units, H. nana eggs were detected in 10.5% of the tested mice by the flotation and in 24.6% by the sedimentation method. A colony of mice was treated against the tapeworm H. nana with praziquantel and emodepside in doses of 2.574 mg praziquantel/100 g body mass and of 0.642 mg emodepside/100 g body mass. The content of the original pipettes (Profender®) was applied as a spot-on on the back of the neck in the area between the shoulders. The application was repeated three times at 14-day intervals. Seven days after the third therapy no H. nana was found in any of the tested mice in the reproductive or the nursery units. After the treatment, cannibalism was no longer observed. This treatment represented one of the steps aimed at improving animal welfare and preventing potential zoonotic disease. The public health significance of this cestode should receive more attention, especially among people who take care of mice, have them as pets, or feed them to reptiles.

Until now there is no evidence that chlamydiosis does exist among cats in the Republic of Croatia regardless of the fact that feline chlamydiosis is a worldwide disease. This report describes the clinical examination of a one year old cat with bilateral conjunctivitis, as well as the diagnostic methods used to confirm infection with Chlamydophila (Cp.) felis. We have used rapid enzyme immunoassay test (EIA) for antigen detection, in order to examine the swabs taken from the eyes and nostrils. The positive result was confirmed by direct immunofluorescence (DIF) made on scrapings from cat's conjunctivas. Conventional polymerase chain reaction (PCR) made on swabs taken from cat's conjunctivas and nostrils and sequencing the PCR product was used to confirmed infection of cat precisely with Chlamydophila felis. No increase of IgG antibodies against chlamydias was noted using indirect immunofluorescence (IFA) method

Uspješnost osposobljavanja budućih doktora veterinarske medicine u izravnoj je vezi s kompetencijama nastavnika, odnosno svih sudionika u osposobljavanju doktorica/ doktora veterinarske medicine iz raznih područja, a koje obuhvaćaju znanje i vještine koje nastoje prenijeti, kao i njihove stavove. Stoga je važno usuglasiti zahtjeve struke u odnosu na kompetencije sudionika u profesionalnom osposobljavanju studenata veterinarske medicine. To se posebno odnosi na vještine koje studenti moraju usvojiti, a odnose se na specifične i generičke kompetencije za obavljanje profesionalnih dužnosti u širem europskom prostoru. Cilj ovog rada je predstaviti „Kompetencije prvoga dana“ (engl. Day One Competences, DOC), odnosno zacrtane nužne profesionalne vještine novih doktorica/doktora veterinarske medicine, kao i pobliže objasniti i olakšati razumijevanje temeljnih odrednica navedenog koncepta. DOC je usvojio Europski koordinacijski odbor za veterinarsku edukaciju (engl. European Coordination Committee on Veterinary Training, ECCVT), koji su osnovali Europska udruga ustanova za veterinarsku naobrazbu (engl. European Association of Establishments for Veterinary Education, EAEVE), Europski odbor za veterinarsku specijalizaciju (engl. European Board of Veterinary Specialisation,EBVS®) i Federacija europskih veterinara (engl. Federation of Veterinarians of Europe, FVE). DOC su detaljno opisane u dokumentu Standardni operativni postupak (SOP, 2019.) Europskog sustava evaluacije veterinarske izobrazbe (engl. European System of Evaluation of Veterinary Training, ESEVT) koji također djeluje unutar EAEVE-a.

A. DOVČ, S. ŽUŽUL, M. BENIĆ, T. MAŠEK, K. STARČEVIĆ: Corticosterone values and blood biochemistry in a model of streptozotocin-induced diabetes: the influence of dietary n6/n3 ratio. Vet. arhiv 89, 885-894, 2019. ABSTRACTThe study was carried out to investigate the influence of dietary n6/n3 ratio in streptozotocin (STZ) induced diabetes on serum biochemistry and corticosterone values in blood in 15 Wistar male rats assigned into three experimental groups, two diabetic and a control group (STZ-N3, STZ-N6, CON) fed with different diets (n6/n3 ratio: ≈ 1, n6/n3 ratio: ≈60, CON n6/n3 ratio: ≈7). Significantly higher values of alkaline phosphatase (AP) and beta-hydroxybutyrate (BHB) and lower levels for triglyceride and albumin were noticed in both STZ treated groups compared to the control. The values for blood urea nitrogen (BUN) were increased in only the STZ-N3 group compared to the control (P<0.05). The values for the total bilirubin (P<0.05) and alanine aminotransferase (ALT) (P<0.05) were higher in only the STZ-N6 group compared to the control. In addition, Vet. arhiv 89 (6), 885-894, 2019 G. Gregurić Gračner et al.: Corticosterone values and blood biochemistry in a model of streptozotocin-induced diabetes: the influence of dietary n6/n3 ratio the STZ-N3 group had lower albumin values compared to the STZ-N6 group (P < 0.001). Corticosterone values did not significantly differ among all three experimental groups indicating similar levels of stress. In conclusion, the n6/n3 ratio significantly influences blood biochemical parameters in STZ-treated rats. Nevertheless, neither the n6/n3 ratio nor blood sampling and handling influences corticosterone values, which proved the validity of the STZ-induced rodent model of diabetes mellitus in the experimental conditions described.