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Zinc oxide nanoparticles-(ZnONPs)modified carbon paste enzyme electrodes (ZnONPsMCPE) were developed for determination of glucose. The determination of glucose was carried out by oxidation of H2O2 at +0.4 V. ZnONPsMCPE provided biocompatible microenvironment for GOx and necessary pathway of electron transfer between GOx and electrode. The response of GOx/ZnONPsMCPE was proportional to glucose concentration and detection limit was 9.1 × 10(-3) mM. Km and Imax, were calculated as 0.124 mM and 2.033 μA. The developed biosensor exhibits high analytical performance with wide linear range (9.1 × 10(-3)-14.5 mM), selectivity and reproducibility. Serum glucose results allow us to ascertain practical utility of GOx/ZnONPsMCPE biosensor.

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