This study is aimed at the theoretical analysis of the acoustic radiation torque and the experimental realization of a controlled rotation of non-spherical particles by ultrasound. A finite element model has been developed and validated to calculate the acoustic radiation torque on a microfiber. The influence of different parameters such as the frequency, fiber size and position in the acoustic field are evaluated. The rotational motion of a non-spherical particle and the resulting drag torque are analyzed as well. This allows for the calculation of the angular velocity of a fiber. Various rotation methods for non-spherical particles with the acoustic radiation torque have been developed, tested experimentally with a microdevice at frequencies in the MHz range and compared to each other. The first method relies on successive change of the wave propagation direction in discrete steps. Three additional rotation methods have been developed which allow for a continuous rotation and alignment at defined orientations. The methods are characterized by the modulation of one single parameter (amplitude, phase or frequency) over time.

Direct collection of extracellular fluid (ECF) plays a central role in the monitoring of neurological disorders. Current approaches using microdialysis catheters are however drastically limited in term of temporal resolution. Here we show a functional in vivo validation of a droplet collection system included at the tip of a neural probe. The system comprises an advanced droplet formation mechanism which enables the collection of neurochemicals present in the brain ECF at high-temporal resolution. The probe was implanted in a rat brain and could successfully collect fluid samples organized in a train of droplets. A microfabricated target plate compatible with most of the surface-based detection methods was specifically developed for sample analysis. The time-resolved brain-fluid samples are analyzed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The results provide a time evolution picture of the cerebral tissues neurochemical composition for selected elements known for their involvement in neurodegenerative diseases.

We propose a novel neural probe which combines microfluidic channels with recording and stimulation electrodes. The developed microfabrication approach enables the concentration of every active element such as electrodes and the sampling inlet in close proximity on the same surface. As a first approach, full functional validation is presented in this work (in vivo testing will be presented in the next study). Electrical characterization by impedance spectroscopy is performed in order to assess the electrode properties. An advanced experimental setup enabling the validation of the fluidic functions of the neural probe is also presented. It allowed the achievement of a high temporal resolution (170 ms) during sampling as a result of the integration of a T-junction droplet generator inside the probe. The droplets reached a volume of 0.84 nL and are separated by a non-aqueous phase (perfluoromethyldecalin, PFD). This probe represents an innovative tool for neuroscientists as it can be implanted in precise brain structures while combining electrical stimulation with sampling at a high temporal resolution.

Contactless rotation of non-spherical particles has been modeled and experimentally achieved using ultrasonic manipulation. For this purpose an acoustic radiation torque was generated by a time-varying pressure field resulting in a change of orientation of the potential well. The rotation method is based on amplitude modulation of two orthogonal ultrasonic modes. The force potential field has been used to evaluate the different modes and actuations to achieve rotation. Experiments have been performed in micro devices with copolymer particles and glass fibers at frequencies in the megahertz range. A continuous rotation was successfully demonstrated and the method allowed to stop the rotation at arbitrary angular positions.

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