Objectives The aim of this study was to detect the rate of follow-up with the latest information and the current level of knowledge held by medical personnel working in different medical fields about Basic Life Support (BLS). Methods The study group consisted of healthcare personnel working the in the province of Izmir. Three groups were designated as the sample groups according to their field of work. The survey questionnaires were prepared in accordance with the updates of Universal BLS guidelines, and they were presented to the study groups during face to face interviews. Results The evaluation of the current knowledge level of the medical personnel about BLS revealed that the knowledge rate of medical personnel on "the rate of cardiac massage and artificial ventilation" was detected as highest, whereas the rate of medical personnel's ability on ranking application steps was the lowest. Knowledge of health professional groups working in the field of pre-hospital emergency care about BLS was found to be statistically significant when compared to that of professional groups working in other medical fields. Conclusions The rate of medical personnels' following up with the latest information about Basic Life Support (BLS) is low. There are differences in the current level of knowledge between sample groups established according to their fields of work.