Bitki biyoçeşitliliğinin korunmasına yönelik biyoteknolojik uygulamalardan biri olan doku kültürü teknikleri özellikle endemik bitkilerin korunmasında sıklıkla kullanılmaktadır. Iridaceae familyasının endemik bir türü olan Iris sari Schott ex Baker, ülkemizde doğal yayılış alanlarına sahiptir. Çalışmamızın amacı, Türkiye'nin geofitleri arasında yer alan Iris sari Schott ex Baker'nin in vitro tekniklerle vejetatif çoğaltımı ve etkili köklendirme olanaklarının araştırılmasıdır. Bu amaçla, Iris sari Schott ex Baker'nin bitkisine ait olgunlaşmamış embriyolar başlangıç eksplantı olarak kullanılmıştır. In vitro çoğaltım çalışmaları sonucunda elde edilen sürgünler ile köklendirme çalışmaları yapılmıştır.
In this study the effects of different sucrose concentrations, and the combinations of jasmonic acid (JA) with auxins (IAA or NAA) or with cytokinin (2iP) on the bulb induction and rooting of in vitro plantlets of Hyacinthus orientalis subsp. chionophyllus Wendelbo, which is endemic in Turkey, were investigated. The effect of four different sucrose concentrations (30, 45, 60 and 90 g L-1) on bulb formation in tissue culture was investigated. These plantlets were cultured on MS medium supplemented with several concentrations and combinations of JA (0.0, 1.0, 2.0 mg L-1) and 2iP (0.0, 0.25 and 0.50 mg L-1), IAA or NAA (0.5, 1.0 mg L-1). In JA- 2iP treatment, the highest number of bulblets (13.7 number/explant) was obtained by the combinations of JA 1.0 mg L-1 + 2iP 0.25 mg L-1. Also, the largest bulblets with the mean diameter of 7.9 mm were found on MS medium supplemented with JA 2.0 mg L-1. In JA – Auxin treatment, the mean root number per bulblet was highest (17.9 number/explant) and root formation rate was maximum (81.14%) on MS medium supplemented with IAA 1.0 mg L-1 + JA 2.0 mg L-1.
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