The ameliorating effects of punicalagin (PUN) were evaluated against some reproductive parameters in male New Zealand White rabbits treated with bisphenol A (BPA). Rabbits (n = 24) were adapted to laboratory conditions for 2 weeks before the experiment. After adaptation, rabbits were randomly assigned into 4 groups. The control group received tap water and corn oil. Rabbits in BPA and PUN groups received 20 mg/kg BPA in corn oil + tap water, and 2 mg/kg PUN in tap water + corn oil, respectively. Rabbits in the last group received the same amounts of BPA and PUN. All treatments were administered by daily oral gavages for 9 weeks. On d 63 of the experiment, semen and blood samples were collected. Blood samples were used for the evaluation of some hormones. After blood collection, rabbits were euthanized and the right testis was stored at –80°C. At the end of the experiment, inhibin, FSH, LH, FSH/LH ratio, FSH/inhibin ratio, and estrogen levels were similar among the groups. However, testosterone levels in serum, and superoxide dismutase, catalase, and malondialdehyde levels in testis tissues were negatively affected by BPA. Moreover, sperm concentrations and the percent of progressive motility significantly declined in BPA treated rabbits. PUN treatment significantly improved these parameters up to control levels when coupled with BPA. The results indicated that PUN has ameliorating effects in neutralizing the possible toxic effects of BPA in male rabbits.
Çalışmada, BPA verilen Yeni Zelanda tavşanlarında punikalajinin bazı oksidan-antioksidan enzimler ile bazı biyokimyasal parametreler üzerine olası etkilerin incelendi. Bu amaçla 2 hafta boyunca laboratuvar koşullarına alıştırılan tavşanlar, her grupta 6 tavşan olacak şekilde rastgele 4 gruba ayrıldı: Kontrol (C; mısır yağı ve distile su), BPA(BPA; mısır yağı içerisinde 20 mg/kg BPA ve distile su), punikalajin (PUN; mısır yağı ve distile su içerisinde 2 mg/kg punikalajin), ve BPA-punikalajin grubu (B+P; mısır yağı içerisinde 20 mg/kg BPA ve distile su içerisinde 2 mg/kg PUN).Uygulamalar 9 hafta boyunca günlük olarak yapıldı ve haftada bir kez yapılan tartımlara göre dozlar ayarlandı. Çalışma sonunda alınan kan ve doku örneklerinden hematolojik, biyokimyasal ve oksidan-antioksidan parametrelerin ölçümleriyapıldı. Analizler neticesinde plazma bilirubin, albümin ve toplam plazma protein düzeyleri ile Mg, P, Ca, Na, K, seviyelerinde herhangi bir istatistiki farka rastlanmazken, farklı gruplardaki plazma, karaciğer ve böbrek glutatyon peroksidaz değerleri de önemsiz bulundu (P>0,1). Oral BPA uygulamaları serum kolesterol, LDL, HDL, amilaz, lipaz, CRP, GGT seviyeleri ile karaciğer ve böbrek dokusundaki malonildialdehit, katalaz ve süperoksit dismütaz seviyelerini olumsuz etkiledi (P
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