The current study was carried out to highlights the antibacterial and antifungal activity of Hedera helix L. by using its different fractions. Both activities were carried out under agar well diffusion method, while for fractions obtaining the cold maceration method was followed which were tested against four fungal species including Fusarium oxysporum, Aspergillus flavors, P. pallidum and Alternaria alternate and four bacterial strains, Staphylococcus aureus, Streptococcus mutans, Serratia marcescens and Methicillin resistance, Staphylococcus aureus. The fractions which were extracted from the Hedera helix shown different zone of inhibition (ZOI) against both the fungal and bacterial strains. Chloroform and n-hexane extracted samples showed the highest antifungal activity against Polyspondylium pallidum with complete inhibition zone and the least activity was shown by an aqueous fraction. In the antibacterial activity, the crude methanolic extracts have shown the highest inhibition zone (46-57%) against the selected bacterial strains, while the lowest activity was reported from aqueous fractions (21-26%). The other fractions moderately inhibit the growth of the selected strains but were significant.

In this paper we have studied the anti-bacterial and anti-fungal potential of Eichhornia crassipes (Mart.) Solms (the common water hyacinth). The main aim was to find a new source against selected bacterial and fungal species was fractionated. Five fractions i.e. n-hexane, aqueous, chloroform, methanolic extracts and ethyl acetate were prepared and tested against four bacterial strains including MRSA (Methicilline resistance staphylococcus aurous), Streptococcus mutansand, Staphylococcus aurous and Serratia marcescense and four fungal strains i.e Alternaria alternate, polyspondylium pallidum, Fusarium oxysporum, Aspergillus flavous. The highest antibacterial activity was displayed by n-hexane fraction (46-48%) and the lowest activity was shown by crude methanolic extracts (38-44%) against all the selected bacterial strains. However in antfungal activities the highest activity was shown by crude methanolic extracts (35-65%) against the four selected fungal species and lowest activity was displayed by the aqueous fractions. The other fractions have also shown significant activites.

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