Background: Ayurveda is a medicinal system which has a long history which goes back to 5000 B.C. Most of the time plant based formulations are used in Ayurveda system of medicine. Chronic kidney disease has been recognized as one of the non communicable diseases in Sri Lanka. Thoravature Taila is a traditional formulation which is mentioned under the Sri Lankan Ayurveda Pharmacopeia and is recommended specially for chronic kidney disease. In ancient time, herbal medicines were standardized based on the organoleptic characters. However, such an evaluation can be vary from individual to individual and is not adequate for industries in large scale. Objective and Methods: Therefore, an attempt was made to standardize Thoravature Taila in terms of physic-chemical parameters and development of Thin Layer Chromatography (TLC). Results: Specific gravity, refractive index, peroxide value, acid value, saponification value iodine value for Thoravature Taila were 0.9580 ± 0.0004, 1.3846± 0.0012, 6.4±0.5 milliequalents/kg, 0.4±0.0 mg KOH/g, 325.5±1.2 mg KOH/g, 16.2± 0.4 g I2100/g respectively. TLC fingerprint of Thoravature Taila was developed using ethyl acetate, methanol, cyclohexane and dichloromethane in a ratio of 0.2:0.2:1.6:3.0 v/v and observed under 254 nm and 366 nm. Conclusion: analytical data such as specific gravity, refractive index, peroxide value, acid value, saponification value and iodine value and TLC fingerprint were developed for Thoravature Taila for the first time and can be considered as the standard parameters when assessing the quality of this medicated oil.